Chromogenic in-situ hybridization (CISH) is a relatively new method for detection of gene amplification using a peroxidase reaction, which can be viewed using a standard light microscope. This.. Introduction. In situ hybridization (ISH) has become an extremely useful tool for the clinical pathology laboratory to aid oncologists, geneticists, and infectious disease specialists in the diagnosis and treatment of their patients. ISH utilizes nucleic acid (DNA or RNA) probes to assess intact cells for various types of genetic alterations.
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Chromogenic in situ hybridisation (CISH) is a powerful method to detect ALKpositive nonsmall
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RNA chromogenic in situ hybridization (CISH) for ciRS7 in various... Download Scientific Diagram
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8. Chromogenic In Situ Hybridization (CISH) CISH enables examination of gene amplification, gene deletion, chromosomal translocations, and chromosomal number. This approach uses conventional peroxidase or alkaline phosphatase reactions using bright-field microscopy on tissues fixed by formalin and embedded in paraffin.. The slides were denatured on a hot plate (94°C) for 3 minutes, and the hybridization was performed overnight at 37°C. After hybridization, the slides were washed with 0.5× standard saline citrate for 5 minutes at 75°C, followed by three washes in PBS/0.2% Tween 20 at room temperature.
